The use of fluorescent probes to assess viability of the plant pathogenic bacterium Clavibacter michiganensis subsp. michiganensis by flow cytometry
نویسندگان
چکیده
منابع مشابه
A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants
Clavibacter michiganensis, subsp. michiganensis is a Gram-positive plant pathogen infecting tomato (Solanum lycopersicum). Despite a considerable economic importance due to significant losses of infected plants and fruits, knowledge about virulence factors of C. michiganensis subsp. michiganensis and host-pathogen interactions on a molecular level are rather limited. In the study presented here...
متن کاملEvidence for plasmid-encoded virulence factors in the phytopathogenic bacterium Clavibacter michiganensis subsp. michiganensis NCPPB382.
The tomato pathogen Clavibacter michiganensis subsp. michiganensis NCPPB382, which causes bacterial wilt, harbors two plasmids pCM1 (27.5 kb) and pCM2 (72 kb). After curing of the plasmids, bacterial derivatives were still proficient in the ability to colonize the host plant and in the production of exopolysaccharides but exhibited a reduced virulence. When one of the two plasmids is lost, ther...
متن کاملThe Genome Sequence of the Tomato - Pathogenic Actinomycete Clavibacter 1 michiganensis subsp . michiganensis NCPPB 382 Reveals a Large Island
4 KARL-HEINZ GARTEMANN, BIRTE ABT, THOMAS BEKEL, ANNETTE BURGER, 5 JUTTA ENGEMANN, MONIKA FLÜGEL, 1 LARS GAIGALAT, ALEXANDER 6 GOESMANN, INES GRÄFEN, JÖRN KALINOWSKI, OLAF KAUP, OLIVER 7 KIRCHNER, LUTZ KRAUSE, BURKHARD LINKE, ALICE McHARDY, FOLKER 8 MEYER,‡ SANDRA POHLE, CHRISTIAN RÜCKERT, 2,3 SUSANNE SCHNEIKER, EVA9 MARIA ZELLERMANN, ALFRED PÜHLER, RUDOLF EICHENLAUB,* OLAF 10 KAISER, AND DANIE...
متن کاملLinear plasmid in the genome of Clavibacter michiganensis subsp. sepedonicus.
Contour-clamped homogeneous electric field gel analysis of genomic DNA of the plant pathogen Clavibacter michiganensis subsp. sepedonicus revealed the presence of a previously unreported extrachromosomal element. This new element was demonstrated to be a linear plasmid. Of 11 strains evaluated, all contained either a 90-kb (pCSL1) or a 140-kb (pCSL2) linear plasmid.
متن کاملStable transformation of the gram-positive phytopathogenic bacterium Clavibacter michiganensis subsp. sepedonicus with several cloning vectors.
In this paper we describe transformation of Clavibacter michiganensis subsp. sepedonicus, the potato ring rot bacterium, with plasmid vectors. Three of the plasmids used, pDM100, pDM302, and pDM306, contain the origin of replication from pCM1, a native plasmid of C. michiganensis subsp. michiganensis. We constructed two new cloning vectors, pHN205 and pHN216, by using the origin of replication ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Fitopatologia Brasileira
سال: 2006
ISSN: 0100-4158
DOI: 10.1590/s0100-41582006000400004